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V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis

In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-A...

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Hauptverfasser: Luo, Yu (VerfasserIn) , Scholl, Stefan (VerfasserIn) , Doering, Anett (VerfasserIn) , Zhang, Yi (VerfasserIn) , Irani, Niloufer G. (VerfasserIn) , Di Rubbo, Simone (VerfasserIn) , Neumetzler, Lutz (VerfasserIn) , Krishnamoorthy, Praveen (VerfasserIn) , Van Houtte, Isabelle (VerfasserIn) , Mylle, Evelien (VerfasserIn) , Bischoff, Volker (VerfasserIn) , Vernhettes, Samantha (VerfasserIn) , Winne, Johan (VerfasserIn) , Friml, Jiří (VerfasserIn) , Stierhof, York-Dieter (VerfasserIn) , Schumacher, Karin (VerfasserIn) , Persson, Staffan (VerfasserIn) , Russinova, Eugenia (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 06 July 2015
In: Nature plants
Year: 2015, Jahrgang: 1, Heft: 7
ISSN:2055-0278
DOI:10.1038/nplants.2015.94
Online-Zugang:Verlag, Volltext: https://doi.org/10.1038/nplants.2015.94
Verlag, Volltext: https://www.nature.com/articles/nplants201594
Volltext
Verfasserangaben:Yu Luo, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer G. Irani, Simone Di Rubbo, Lutz Neumetzler, Praveen Krishnamoorthy, Isabelle Van Houtte, Evelien Mylle, Volker Bischoff, Samantha Vernhettes, Johan Winne, Jiří Friml, York-Dieter Stierhof, Karin Schumacher, Staffan Persson and Eugenia Russinova
Beschreibung
Zusammenfassung:In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
Beschreibung:Gesehen am 14.08.2019
Beschreibung:Online Resource
ISSN:2055-0278
DOI:10.1038/nplants.2015.94

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