Isolation of myricitrin and 3,5-di-O-methyl gossypetin from Syzygium samarangense and evaluation of their involvement in protecting keratinocytes against oxidative stress via activation of the Nrf-2 pathway
The wax apple (Syzygium samarangense) is traditionally employed as an antibacterial and immunostimulant drug in traditional medicine. This plant is rich in different flavonoids and tannins. In this study, we isolated two compounds from S. samarangense leaves: myricitrin and 3,5-di-O-methyl gossypeti...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
13 May 2019
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| In: |
Molecules
Year: 2019, Volume: 24, Issue: 9 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24091839 |
| Online Access: | Verlag, Volltext: https://doi.org/10.3390/molecules24091839 Verlag, Volltext: https://www.mdpi.com/1420-3049/24/9/1839 
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| Author Notes: | Mansour Sobeh, Ganna Petruk, Samir Osman, Mohamed A. El Raey, Paola Imbimbo, Daria Maria Monti and Michael Wink |
| Summary: | The wax apple (Syzygium samarangense) is traditionally employed as an antibacterial and immunostimulant drug in traditional medicine. This plant is rich in different flavonoids and tannins. In this study, we isolated two compounds from S. samarangense leaves: myricitrin and 3,5-di-O-methyl gossypetin. Then, we investigated the mechanisms of action of the two compounds against oxidative stress (induced by sodium arsenite) and inflammation (induced by UV light) on human keratinocytes. We could clearly demonstrate that the pre-treatment of cells with both compounds was able to mitigate the negative effects induced by oxidative stress, as no alteration in reactive oxygen species (ROS) production, glutathione (GSH) level, or protein oxidation was observed. Additionally, both compounds were able to modulate mitogen-activated protein kinase (MAPK) signaling pathways to counteract oxidative stress activation. Finally, we showed that 3,5-di-O-methyl gossypetin exerted its antioxidant activity through the nuclear transcription factor-2 (Nrf-2) pathway, stimulating the expression of antioxidant proteins, such as HO-1 and Mn-SOD-3. |
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| Item Description: | Gesehen am 20.08.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24091839 |