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A comprehensive toolkit for inducible, cell type-specific gene expression in arabidopsis

Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell type-specific transactivation in Arabidopsis (Arabidopsis thaliana) based on the well-established combination of the chimeric GR-LhG4 transcription f...

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Main Authors: Schürholz, Anne-Kathrin (Author) , López-Salmerón, Vadir (Author) , Li, Zhenni (Author) , Forner, Joachim (Author) , Wenzl, Christian (Author) , Gaillochet, Christophe (Author) , Augustin, Sebastian (Author) , Vilches-Barro, Amaya (Author) , Fuchs, Michael (Author) , Gebert, Michael (Author) , Lohmann, Jan U. (Author) , Greb, Thomas (Author) , Wolf, Sebastian (Author)
Format: Article (Journal)
Language:English
Published: September 2018
In: Plant physiology
Year: 2018, Volume: 178, Issue: 1, Pages: 40-53
ISSN:1532-2548
DOI:10.1104/pp.18.00463
Online Access:Verlag, Volltext: https://doi.org/10.1104/pp.18.00463
Verlag, Volltext: http://www.plantphysiol.org/content/178/1/40
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Author Notes:Ann-Kathrin Schürholz, Vadir López-Salmerón, Zhenni Li, Joachim Forner, Christian Wenzl, Christophe Gaillochet, Sebastian Augustin, Amaya Vilches Barro, Michael Fuchs, Michael Gebert, Jan U. Lohmann, Thomas Greb, and Sebastian Wolf
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Summary:Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell type-specific transactivation in Arabidopsis (Arabidopsis thaliana) based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate cloning system, we produced a comprehensive set of transgenic lines termed GR-LhGf lsw 4 driver lines targeting most tissues in the Arabidopsis shoot and root with a strong focus on the indeterminate meristems. When we combined these transgenic lines with effectors under the control of the pOp promoter, we observed tight temporal and spatial control of gene expression. In particular, inducible expression in F1 plants obtained from crosses of driver and effector lines allows for rapid assessment of the cell type-specific impact of an effector with high temporal resolution. Thus, our comprehensive and flexible method is suitable for overcoming the limitations of ubiquitous genetic approaches, the outputs of which often are difficult to interpret due to the widespread existence of compensatory mechanisms and the integration of diverging effects in different cell types.
Item Description:Gesehen am 22.08.2019
Physical Description:Online Resource
ISSN:1532-2548
DOI:10.1104/pp.18.00463

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