Neuroprotection and antioxidative effects of Sijunzi Tang Decoction in the nematode Caenorhabditis elegans
Introduction - Knowledge about the pharmacological properties of traditional TCM formulas is scarce as is their impact on aging. This study evaluated the potential effects of Sijunzi Tang Decoction (SJZTD), Si-Wu-Tang Decoction (SWTD), and Liuwei Dihuang Decoction (LWDHD) against oxidative stress an...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 2016
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| In: |
European journal of integrative medicine
Year: 2016, Volume: 8, Issue: 4, Pages: 526-532 |
| ISSN: | 1876-3839 |
| DOI: | 10.1016/j.eujim.2016.03.009 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.eujim.2016.03.009 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1876382016300257 
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| Author Notes: | Andrea Schlotterer, Henry Johannes Greten, Bjoern Andrew Remppis, Georgi Kukudov, Thomas Efferth, Jorge Machado, Per Humpert, Hans-Peter Hammes, Michael Morcos |
| Summary: | Introduction - Knowledge about the pharmacological properties of traditional TCM formulas is scarce as is their impact on aging. This study evaluated the potential effects of Sijunzi Tang Decoction (SJZTD), Si-Wu-Tang Decoction (SWTD), and Liuwei Dihuang Decoction (LWDHD) against oxidative stress and glucotoxic stress-induced neuronal dysfunction and lifespan reduction. - Methods - Nematodes (C. elegans) were cultivated under 10mmol/L paraquat or 400mmol/L glucose to assess the protective effects of a systemic Traditional Chinese Medicine (TCM) treatment. Lifespan was determined by calculation of Kaplan-Meier survival curves, formation of reactive oxygen species was quantified by hydroethidine staining, and neuronal functions were evaluated by worm tracking analysis. - Results - A 10% SJZTD solution largely normalized nematode survival under both oxidative stress (increased 3-day-survival rate by 143%) and glucotoxic stress (extended mean lifespan from 19.90 to 23.71 days). Under high glucose conditions we demonstrated both antioxidative effects (reduced Reactive Oxygen Species (ROS) formation by 71%) and neuroprotective effects (improved whole nematode motility by 179%). SWTD and LWDHD failed to confer protection in our model system. - Conclusions - The neuroprotective effects for SJZT decoction against glucotoxic stress due to pronounced antioxidant properties were demonstrated. Future studies should be extended to protection of the neurovascular system in higher organisms. |
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| Item Description: | Gesehen am 23.08.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1876-3839 |
| DOI: | 10.1016/j.eujim.2016.03.009 |