MicroRNA-365a-3p inhibits c-Rel-mediated NF-[kappa] B signaling and the progression of pancreatic cancer
NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
23 March 2019
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| In: |
Cancer letters
Year: 2019, Volume: 452, Pages: 203-212 |
| ISSN: | 1872-7980 |
| DOI: | 10.1016/j.canlet.2019.03.025 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.canlet.2019.03.025 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0304383519301818 
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| Author Notes: | Libo Yin, Xi Xiao, Christina Georgikou, Yefeng Yin, Li Liu, Svetlana Karakhanova, Yiqiao Luo, Jury Gladkich, Joerg Fellenberg, Carsten Sticht, Norbert Gretz, Wolfgang Gross, Ingrid Herr |
| Summary: | NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we predicted miR-365a as the top candidate for the sulforaphane-induced inhibition of the NF-κB subunit c-Rel. The lipofection of miR-365a-3p mimics inhibited the luciferase activity of a c-Rel 3′-UTR construct, as well as c-Rel expression, NF-κB activity, and tumor viability, migration, and clonogenicity, whereas apoptosis was induced. In vivo, miR-365a-3p reduced the volume of tumor xenografts and the expression of progression markers. In a tissue array, the expression of miR-365a-3p was absent in almost all 91 malignant tissues but not in 5 normal tissues, thus confirming the previous results. Our observations suggest that sulforaphane-induced miR-365a-3p expression inhibits NF-κB activity by downregulating c-Rel, which prevents the progression of PDA. |
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| Item Description: | Im Titel ist "kappa" als griechischer Buchstabe dargestellt Gesehen am 12.09.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1872-7980 |
| DOI: | 10.1016/j.canlet.2019.03.025 |