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MicroRNA-365a-3p inhibits c-Rel-mediated NF-[kappa] B signaling and the progression of pancreatic cancer

NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we...

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Hauptverfasser: Yin, Libo (VerfasserIn) , Xiao, Xi (VerfasserIn) , Georgikou, Christina (VerfasserIn) , Yin, Yefeng (VerfasserIn) , Liu, Li (VerfasserIn) , Karakhanova, Svetlana (VerfasserIn) , Luo, Yiqiao (VerfasserIn) , Gladkich, Jury (VerfasserIn) , Fellenberg, Jörg (VerfasserIn) , Sticht, Carsten (VerfasserIn) , Gretz, Norbert (VerfasserIn) , Gross, Wolfgang (VerfasserIn) , Herr, Ingrid (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 23 March 2019
In: Cancer letters
Year: 2019, Jahrgang: 452, Pages: 203-212
ISSN:1872-7980
DOI:10.1016/j.canlet.2019.03.025
Online-Zugang:Verlag, Volltext: https://doi.org/10.1016/j.canlet.2019.03.025
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0304383519301818
Volltext
Verfasserangaben:Libo Yin, Xi Xiao, Christina Georgikou, Yefeng Yin, Li Liu, Svetlana Karakhanova, Yiqiao Luo, Jury Gladkich, Joerg Fellenberg, Carsten Sticht, Norbert Gretz, Wolfgang Gross, Ingrid Herr
Beschreibung
Zusammenfassung:NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we predicted miR-365a as the top candidate for the sulforaphane-induced inhibition of the NF-κB subunit c-Rel. The lipofection of miR-365a-3p mimics inhibited the luciferase activity of a c-Rel 3′-UTR construct, as well as c-Rel expression, NF-κB activity, and tumor viability, migration, and clonogenicity, whereas apoptosis was induced. In vivo, miR-365a-3p reduced the volume of tumor xenografts and the expression of progression markers. In a tissue array, the expression of miR-365a-3p was absent in almost all 91 malignant tissues but not in 5 normal tissues, thus confirming the previous results. Our observations suggest that sulforaphane-induced miR-365a-3p expression inhibits NF-κB activity by downregulating c-Rel, which prevents the progression of PDA.
Beschreibung:Im Titel ist "kappa" als griechischer Buchstabe dargestellt
Gesehen am 12.09.2019
Beschreibung:Online Resource
ISSN:1872-7980
DOI:10.1016/j.canlet.2019.03.025

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