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Gene expression atlas of a developing tissue by single cell expression correlation analysis

The Drosophila wing disc has been a fundamental model system for the discovery of key signaling pathways and for our understanding of developmental processes. However, a complete map of gene expression in this tissue is lacking. To obtain a gene expression atlas in the wing disc, we employed single...

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Bibliographic Details
Main Authors: Bageritz, Josephine (Author) , Willnow, Philipp (Author) , Valentini, Erica (Author) , Leible, Svenja (Author) , Boutros, Michael (Author) , Teleman, Aurelio A. (Author)
Format: Article (Journal)
Language:English
Published: 29 July 2019
In: Nature methods
Year: 2019, Volume: 16, Issue: 8, Pages: 750-756
ISSN:1548-7105
DOI:10.1038/s41592-019-0492-x
Online Access:Verlag, Volltext: https://doi.org/10.1038/s41592-019-0492-x
Verlag, Volltext: https://www.nature.com/articles/s41592-019-0492-x
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Author Notes:Josephine Bageritz, Philipp Willnow, Erica Valentini, Svenja Leible, Michael Boutros and Aurelio A. Teleman
Description
Summary:The Drosophila wing disc has been a fundamental model system for the discovery of key signaling pathways and for our understanding of developmental processes. However, a complete map of gene expression in this tissue is lacking. To obtain a gene expression atlas in the wing disc, we employed single cell RNA sequencing (scRNA-seq) and developed a method for analyzing scRNA-seq data based on gene expression correlations rather than cell mapping. This enables us to compute expression maps for all detected genes in the wing disc and to discover 824 genes with spatially restricted expression patterns. This approach identifies clusters of genes with similar expression patterns and functional relevance. As proof of concept, we characterize the previously unstudied gene CG5151 and show that it regulates Wnt signaling. Our method will enable the leveraging of scRNA-seq data for generating expression atlases of undifferentiated tissues during development.
Item Description:Gesehen am 12.09.2019
Physical Description:Online Resource
ISSN:1548-7105
DOI:10.1038/s41592-019-0492-x

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