Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata)
Avian uropygial glands have received increasing attention in recent years, but little is known about micro-organisms in uropygial glands. In this study, we isolated a strain of Gram-stain-positive, non-motile, non-spore-forming cocci, designated 442<span class="jp-sup">T</span>...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
10 January 2019
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| In: |
International journal of systematic and evolutionary microbiology
Year: 2019, Volume: 69, Issue: 2, Pages: 447-451 |
| ISSN: | 1466-5034 |
| DOI: | 10.1099/ijsem.0.003170 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1099/ijsem.0.003170 Verlag: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003170 
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| Author Notes: | Markus Santhosh Braun, Erjia Wang, Stefan Zimmermann, Hermann Wagner, Michael Wink |
| Summary: | Avian uropygial glands have received increasing attention in recent years, but little is known about micro-organisms in uropygial glands. In this study, we isolated a strain of Gram-stain-positive, non-motile, non-spore-forming cocci, designated 442<span class="jp-sup">T</span>, from the uropygial gland of an American barn owl (<em class="">Tyto furcata</em>) and characterized it using a polyphasic approach. 16S rRNA gene sequence analysis placed the isolate in the genus <em class=""> <span class="named-content-genus"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5886" rel="namesforlife-name" target="xrefwindow" title="Kocuria">Kocuria</a> </span></em>. The G+C content was 70.8 mol%, the major menaquinone was MK-7(H<sub class="">2</sub>) and the predominant cellular fatty acids were anteiso-C<sub class="">15:0</sub>, anteiso-C<sub class="">17:0</sub> and iso-C<sub class="">15:0</sub>. Phylogenetic analyses based on the 16S rRNA gene identified <em class=""> <span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5892" rel="namesforlife-name" target="xrefwindow" title="Kocuria rhizophila">Kocuria rhizophila</a></span></em> DSM 11926<span class="jp-sup">T</span> (99.6% similarity), <em class=""><span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.21139" rel="namesforlife-name" target="xrefwindow" title="Kocuria salsicia">Kocuria salsicia</a></span></em> DSM 24776<span class="jp-sup">T</span> (98.7%), <em class=""> <span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5893" rel="namesforlife-name" target="xrefwindow" title="Kocuria varians">Kocuria varians</a></span></em> DSM 20033<span class="jp-sup">T</span> (98.3%) and <em class=""><span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.8647" rel="namesforlife-name" target="xrefwindow" title="Kocuria marina">Kocuria marina</a> </span> </em> DSM 16420<span class="jp-sup">T</span> (98.3%) as the most closely related species. However, average nucleotide identity values below 86% indicated that the isolate differed from all species hitherto described. Chemotaxonomic analyses and whole-cell protein profiles corroborated these findings. Accordingly, the isolate is considered to be a member of a novel species, for which the name <em class=""><span class="named-content-genus"><a class="namesforlife" href="https://doi.org/10.1601/nm.5886" rel="namesforlife-name" target="xrefwindow" title="Kocuria">Kocuria</a></span> tytonis</em> sp. nov. is proposed. The type strain is 442<span class="jp-sup">T</span> (=DSM 104130<span class="jp-sup">T</span>=LMG 29944<span class="jp-sup">T</span>)., |
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| Item Description: | Gesehen am 19.11.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1466-5034 |
| DOI: | 10.1099/ijsem.0.003170 |