Scurfy mice develop features of connective tissue disease overlap syndrome and mixed connective tissue disease in the absence of regulatory T cells
Due to a missense mutation in the Foxp3 gene, scurfy mice are deficient in functional regulatory T cells (Treg). The consequent loss of peripheral tolerance manifests itself by fatal autoimmune mediated multi-organ disease. Previous studies have outlined the systemic inflammatory disease and demonst...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
24 April 2019
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| In: |
Frontiers in immunology
Year: 2019, Jahrgang: 10 |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2019.00881 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.3389/fimmu.2019.00881 Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2019.00881/full 
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| Verfasserangaben: | Osman K. Yilmaz, Stefanie Haeberle, Meifeng Zhang, Marvin J. Fritzler, Alexander H. Enk and Eva N. Hadaschik |
| Zusammenfassung: | Due to a missense mutation in the Foxp3 gene, scurfy mice are deficient in functional regulatory T cells (Treg). The consequent loss of peripheral tolerance manifests itself by fatal autoimmune mediated multi-organ disease. Previous studies have outlined the systemic inflammatory disease and demonstrated production of anti-nuclear antibodies (ANA) in scurfy mice. However, specific autoantibody targets remained to be defined. ANA are immunological markers for several connective tissue diseases (CTD) and target a large number of intracellular molecules. Therefore, we examined scurfy sera for the presence of different ANA specificities and further assessed the organ involvement in these animals. Indirect immunofluorescence was used as a screen for ANA in the sera of scurfy mice and dilutions of 1/100 were considered positive. Addressable laser bead immunoassays (ALBIA) were used to detect specific autoantibody targets. Subsequent histological tissue evaluation was verified by hematoxylin and eosin (H&E) staining. In our study, we observed that nearly all scurfy mice produced ANA. The most prevalent pattern in scurfy sera was nuclear coarse speckled, also known as the AC-5 pattern according to the International Consensus on ANA Patterns. U1-ribonucleoprotein (U1RNP) was found to be the most common target antigen recognized by autoantibodies in scurfy mice. Additionally, scurfy mice exhibited a mild myositis with histological characteristics similar to polymyositis/dermatomyositis. Myopathy-specific autoantibody profile revealed significantly increased levels of anti-SMN (survival of motor neuron) as well as anti-Gemin3 antibodies in scurfy sera. Overall, we demonstrate that the impaired peripheral tolerance in the absence of regulatory T cells in scurfy mice is associated with features of mixed connective tissue disease (MCTD). This includes, along with our previous findings, very high titers of anti-U1RNP antibodies and an inflammatory myopathy. |
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| Beschreibung: | Gesehen am 07.10.2019 |
| Beschreibung: | Online Resource |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2019.00881 |