Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently t...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
06 September 2019
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| In: |
Nature Communications
Year: 2019, Volume: 10 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12046-3 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1038/s41467-019-12046-3 Verlag: https://www.nature.com/articles/s41467-019-12046-3 
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| Author Notes: | Bojana Lucic, Heng-Chang Chen, Maja Kuzman, Eduard Zorita, Julia Wegner, Vera Minneker, Wei Wang, Raffaele Fronza, Stefanie Laufs, Manfred Schmidt, Ralph Stadhouders, Vassilis Roukos, Kristian Vlahovicek, Guillaume J. Filion & Marina Lusic |
| Summary: | HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are proximal to super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results provide evidence of the relevance of the spatial compartmentalization of the genome for HIV-1 integration, thus further strengthening the role of nuclear architecture in viral infection. |
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| Item Description: | Gesehen am 07.10.2019 |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12046-3 |