Cover Image

Site-to-site reproducibility and spatial resolution in MALDI-MSI of peptides from formalin-fixed paraffin-embedded samples

Purpose To facilitate the transition of MALDI-MS Imaging (MALDI-MSI) from basic science to clinical application, it is necessary to analyze formalin-fixed paraffin-embedded (FFPE) tissues. The aim is to improve in situ tryptic digestion for MALDI-MSI of FFPE samples and determine if similar results...

Full description

Saved in:
Bibliographic Details
Main Authors: Ly, Alice (Author) , Longuespée, Rémi (Author) , Marsching, Christian (Author) , Kriegsmann, Katharina (Author) , Hopf, Carsten (Author) , Schirmacher, Peter (Author) , Kriegsmann, Mark (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: Proteomics. Clinical applications
Year: 2018, Volume: 13, Issue: 1
ISSN:1862-8354
DOI:10.1002/prca.201800029
Online Access:Verlag, Volltext: https://doi.org/10.1002/prca.201800029
Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800029
Get full text
Author Notes:Alice Ly, Rémi Longuespée, Rita Casadonte, Petra Wandernoth, Kristina Schwamborn, Christine Bollwein, Christian Marsching, Katharina Kriegsmann, Carsten Hopf, Wilko Weichert, Jörg Kriegsmann, Peter Schirmacher, Mark Kriegsmann, and Sören-Oliver Deininger
Description
Summary:Purpose To facilitate the transition of MALDI-MS Imaging (MALDI-MSI) from basic science to clinical application, it is necessary to analyze formalin-fixed paraffin-embedded (FFPE) tissues. The aim is to improve in situ tryptic digestion for MALDI-MSI of FFPE samples and determine if similar results would be reproducible if obtained from different sites. Experimental Design FFPE tissues (mouse intestine, human ovarian teratoma, tissue microarray of tumor entities sampled from three different sites) are prepared for MALDI-MSI. Samples are coated with trypsin using an automated sprayer then incubated using deliquescence to maintain a stable humid environment. After digestion, samples are sprayed with CHCA using the same spraying device and analyzed with a rapifleX MALDI Tissuetyper at 50 µm spatial resolution. Data are analyzed using flexImaging, SCiLS, and R. Results Trypsin application and digestion are identified as sources of variation and loss of spatial resolution in the MALDI-MSI of FFPE samples. Using the described workflow, it is possible to discriminate discrete histological features in different tissues and enabled different sites to generate images of similar quality when assessed by spatial segmentation and PCA. Conclusions and Clinical Relevance Spatial resolution and site-to-site reproducibility can be maintained by adhering to a standardized MALDI-MSI workflow.
Item Description:First published: 08 November 2018
Gesehen am 24.10.2019
Physical Description:Online Resource
ISSN:1862-8354
DOI:10.1002/prca.201800029

Similar Items