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CD7 is expressed on a subset of normal CD34-positive myeloid precursors

Objective To improve monitoring of myeloid neoplasms by flow cytometry-based minimal residual disease (MRD) analysis, we analyzed the significance of leukemia-associated immunophenotype (LAIP) markers in 44 patients. Methods In a pilot study cohort, peripheral blood or bone marrow samples from 13 pa...

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Main Authors: Kriegsmann, Katharina (Author) , Löffler, Harald (Author) , Eckstein, Volker (Author) , Schulz, Renate (Author) , Kräker, Sandra (Author) , Braun, Ute (Author) , Luft, Thomas (Author) , Hegenbart, Ute (Author) , Schönland, Stefan (Author) , Dreger, Peter (Author) , Krämer, Alwin (Author) , Ho, Anthony Dick (Author) , Müller-Tidow, Carsten (Author) , Hundemer, Michael (Author)
Format: Article (Journal)
Language:English
Published: 24 May 2018
In: European journal of haematology
Year: 2018, Volume: 101, Issue: 3, Pages: 318-325
ISSN:1600-0609
DOI:10.1111/ejh.13100
Online Access:Verlag, Volltext: https://doi.org/10.1111/ejh.13100
Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1111/ejh.13100
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Author Notes:Katharina Kriegsmann, Harald Löffler, Volker Eckstein, Renate Schulz, Sandra Kräker, Ute Braun, Thomas Luft, Ute Hegenbart, Stefan Schönland, Peter Dreger, Alwin Krämer, Anthony D. Ho, Carsten Müller‐Tidow, Michael Hundemer
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Summary:Objective To improve monitoring of myeloid neoplasms by flow cytometry-based minimal residual disease (MRD) analysis, we analyzed the significance of leukemia-associated immunophenotype (LAIP) markers in 44 patients. Methods In a pilot study cohort, peripheral blood or bone marrow samples from 13 patients with myeloid neoplasms and one case of B lymphoblastic leukemia in complete hematologic remission after allogeneic bone marrow or stem cell transplantation were subjected to selection for leukemia-specific phenotypes by fluorescence-activated cell sorting using individual marker combinations, followed by PCR-based chimerism analysis. Results The feasibility of this method could be demonstrated, with selection being successful in 12 cases, including two cases where mixed chimerism was found exclusively in sorted cells. Interestingly, four specimens displayed full donor chimerism in cells expressing the presumably aberrant combination CD34+/CD7+. Further analyses, including assessment of an independent cohort of 25 patients not affected by neoplastic bone marrow infiltration, revealed that normal myeloid precursors usually include a population coexpressing CD34, CD13, CD33, and CD7. Conclusion We conclude that the combination CD34+/CD7+ might not be suitable as an LAIP for MRD diagnostics and that a subset of normal myeloid precursors in the bone marrow expresses CD7.
Item Description:Gesehen am 29.10.2019
Physical Description:Online Resource
ISSN:1600-0609
DOI:10.1111/ejh.13100

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