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Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism

Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrat...

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Main Authors: Mitterer, Valentin (Author) , Shayan, Ramtin (Author) , Ferreira-Cerca, Sébastien (Author) , Murat, Guillaume (Author) , Enne, Tanja (Author) , Rinaldi, Dana (Author) , Weigl, Sarah (Author) , Omanic, Hajrija (Author) , Gleizes, Pierre-Emmanuel (Author) , Kressler, Dieter (Author) , Plisson-Chastang, Celia (Author) , Pertschy, Brigitte (Author)
Format: Article (Journal)
Language:English
Published: 21 June 2019
In: Nature Communications
Year: 2019, Volume: 10
ISSN:2041-1723
Online Access: Get full text
Author Notes:Valentin Mitterer, Ramtin Shayan, Sebastien Ferreira-Cerca, Guillaume Murat, Tanja Enne, Dana Rinaldi, Sarah Weigl, Hajrija Omanic, Pierre-Emmanuel Gleizes, Dieter Kressler, Celia Plisson-Chastang & Brigitte Pertschy
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Summary:Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.
Item Description:Published: 21 June 2019
Gesehen am 31.10.2019
Physical Description:Online Resource
ISSN:2041-1723

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