A genetic interaction map of cell cycle regulators
Cell-based RNA interference (RNAi) is a powerful approach to screen for modulators of many cellular processes. However, resulting candidate gene lists from cell-based assays comprise diverse effectors, both direct and indirect, and further dissecting their functions can be challenging. Here we scree...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 24, 2016
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| In: |
Molecular biology of the cell
Year: 2016, Volume: 27, Issue: 8, Pages: 1397-1407 |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E15-07-0467 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1091/mbc.E15-07-0467 Verlag, Volltext: https://www.molbiolcell.org/doi/10.1091/mbc.E15-07-0467 
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| Author Notes: | Maximilian Billmann, Thomas Horn, Bernd Fischer, Thomas Sandmann, Wolfgang Huber, and Michael Boutros |
| Summary: | Cell-based RNA interference (RNAi) is a powerful approach to screen for modulators of many cellular processes. However, resulting candidate gene lists from cell-based assays comprise diverse effectors, both direct and indirect, and further dissecting their functions can be challenging. Here we screened a genome-wide RNAi library for modulators of mitosis and cytokinesis in Drosophila S2 cells. The screen identified many previously known genes as well as modulators that have previously not been connected to cell cycle control. We then characterized ∼300 candidate modifiers further by genetic interaction analysis using double RNAi and a multiparametric, imaging-based assay. We found that analyzing cell cycle-relevant phenotypes increased the sensitivity for associating novel gene function. Genetic interaction maps based on mitotic index and nuclear size grouped candidates into known regulatory complexes of mitosis or cytokinesis, respectively, and predicted previously uncharacterized components of known processes. For example, we confirmed a role for the Drosophila CCR4 mRNA processing complex component l(2)NC136 during the mitotic exit. Our results show that the combination of genome-scale RNAi screening and genetic interaction analysis using process-directed phenotypes provides a powerful two-step approach to assigning components to specific pathways and complexes. |
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| Item Description: | Gesehen am 07.11.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E15-07-0467 |