Paramyxoviruses for tumor-targeted immunomodulation: design and evaluation ex vivo
Successful cancer immunotherapy has the potential to achieve long-term tumor control. Despite recent clinical successes, there remains an urgent need for safe and effective therapies tailored to individual tumor immune profiles. Oncolytic viruses enable the induction of anti-tumor immune responses a...
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| Main Authors: | , |
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| Format: | Article (Journal) Video |
| Language: | English |
| Published: |
1/07/2019
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| In: |
JoVE. Video journal
Year: 2019, Issue: 143, Pages: ? |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58651 |
| Subjects: | |
| Online Access: | Verlag, Volltext: https://doi.org/10.3791/58651 Verlag: https://www.jove.com/video/58651/paramyxoviruses-for-tumor-targeted-immunomodulation-design-evaluation 
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| Author Notes: | Johannes P.W. Heidbuechel, Christine E. Engeland |
| Summary: | Successful cancer immunotherapy has the potential to achieve long-term tumor control. Despite recent clinical successes, there remains an urgent need for safe and effective therapies tailored to individual tumor immune profiles. Oncolytic viruses enable the induction of anti-tumor immune responses as well as tumor-restricted gene expression. This protocol describes the generation and ex vivo analysis of immunomodulatory oncolytic vectors. Focusing on measles vaccine viruses encoding bispecific T cell engagers as an example, the general methodology can be adapted to other virus species and transgenes. The presented workflow includes the design, cloning, rescue, and propagation of recombinant viruses. Assays to analyze replication kinetics and lytic activity of the vector as well as functionality of the isolated immunomodulator ex vivo are included, thus facilitating the generation of novel agents for further development in preclinical models and ultimately clinical translation. |
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| Item Description: | Enthält auch Versuchsbeschreibung in Textform Gesehen am 13.11.2019 Wissenschaftlicher Film. Deutschland. 2019 |
| Physical Description: | Online Resource |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58651 |