Switchable release of bone morphogenetic protein from thermoresponsive poly(NIPAM-co-DMAEMA)/cellulose sulfate particle coatings
Thermoresponsive coatings of poly(N-isopropylacrylamide-co-DMAEMA)/cellulose sulfate (PNIPAM-DMAEMA/CS) complexes are reported eluting bone-morphogenetic-protein-2 (BMP-2) on demand relevant for implant assisted local bone healing. PNIPAM-DMAEMA/CS dispersions contained colloid particles with hydrod...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 November 2018
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| In: |
Polymers
Year: 2018, Volume: 10, Issue: 12 |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym10121314 |
| Online Access: | Verlag, Volltext: https://doi.org/10.3390/polym10121314 Verlag, Volltext: https://www.mdpi.com/2073-4360/10/12/1314 
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| Author Notes: | Martin Müller, Birgit Urban, Berthold Reis, Xiaoqian Yu, Anna Luise Grab, Elisabetta Ada Cavalcanti-Adam and Dirk Kuckling |
| Summary: | Thermoresponsive coatings of poly(N-isopropylacrylamide-co-DMAEMA)/cellulose sulfate (PNIPAM-DMAEMA/CS) complexes are reported eluting bone-morphogenetic-protein-2 (BMP-2) on demand relevant for implant assisted local bone healing. PNIPAM-DMAEMA/CS dispersions contained colloid particles with hydrodynamic radii RH = 170-288 nm at T = 25 °C shrinking to RH = 74-103 nm at T = 60 °C. Obviously, PNIPAM-DMAEMA/CS undergoes volume phase transition (VPT) analogously to pure PNIPAM, when critical VPT temperature (VPTT) is exceeded. Temperature dependent turbidity measurements revealed broad VPT and VPTT 47 °C for PNIPAM-DMAEMA/CS colloid dispersions at pH = 7.0. FTIR spectroscopy on thermoresponsive PNIPAM-DMAEMA/CS particle coatings at germanium model substrates under HEPES buffer indicated both wet-adhesiveness and VPT behavior based on diagnostic band intensity increases with temperature. From respective temperature courses empirical VPTT ≈ 42 °C for PNIPAM-DMAEMA/CS coatings at pH = 7.0 were found, which were comparable to VPTT found for respective dispersions. Finally, the PNIPAM-DMAEMA/CS coatings were loaded with BMP-2 and model protein papain (PAP). Time dependent FTIR spectroscopic measurements showed, that for T = 37 °C there was a relative protein release of ≈ 30% for PAP and ≈ 10% for BMP-2 after 24 h, which did not increase further. Heating to T = 42 °C for PAP and to 47 °C for BMP-2 further secondary protein release of ≈ 20% after 24 h was found, respectively, interesting for clinical applications. BMP-2 eluted even at 47 °C was found to be still biologically active. |
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| Item Description: | Gesehen am 27.11.2019 |
| Physical Description: | Online Resource |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym10121314 |