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Correlated magnetic resonance imaging and ultramicroscopy (MR-UM) is a tool kit to assess the dynamics of glioma angiogenesis

Neoangiogenesis is a pivotal therapeutic target in glioblastoma. Tumor monitoring requires imaging methods to assess treatment effects and disease progression. Until now mapping of the tumor vasculature has been difficult. We have developed a combined magnetic resonance and optical toolkit to study...

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Main Authors: Breckwoldt, Michael O. (Author) , Kurz, Felix T. (Author) , Hoffmann, Angelika (Author) , Sahm, Katharina (Author) , Ott, Martina (Author) , Deumelandt, Katrin (Author) , Schwarz, Daniel (Author) , Fischer, Manuel (Author) , Milford, David (Author) , Solecki, Gergely (Author) , Chiblak, Sara (Author) , Abdollahi, Amir (Author) , Winkler, Frank (Author) , Wick, Wolfgang (Author) , Platten, Michael (Author) , Heiland, Sabine (Author) , Bendszus, Martin (Author) , Tews, Björn (Author)
Format: Article (Journal)
Language:English
Published: 02 February 2016
In: eLife
Year: 2016, Volume: 5
ISSN:2050-084X
DOI:10.7554/eLife.11712
Online Access:Verlag, Volltext: https://doi.org/10.7554/eLife.11712
Verlag: https://elifesciences.org/articles/11712
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Author Notes:Michael O Breckwoldt, Julia Bode, Felix T Kurz, Angelika Hoffmann, Katharina Ochs, Martina Ott, Katrin Deumelandt, Thomas Krüwel, Daniel Schwarz, Manuel Fischer, Xavier Helluy, David Milford, Klara Kirschbaum, Gergely Solecki, Sara Chiblak, Amir Abdollahi, Frank Winkler, Wolfgang Wick, Michael Platten, Sabine Heiland, Martin Bendszus, Björn Tews
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Summary:Neoangiogenesis is a pivotal therapeutic target in glioblastoma. Tumor monitoring requires imaging methods to assess treatment effects and disease progression. Until now mapping of the tumor vasculature has been difficult. We have developed a combined magnetic resonance and optical toolkit to study neoangiogenesis in glioma models. We use in vivo magnetic resonance imaging (MRI) and correlative ultramicroscopy (UM) of ex vivo cleared whole brains to track neovascularization. T2* imaging allows the identification of single vessels in glioma development and the quantification of neovessels over time. Pharmacological VEGF inhibition leads to partial vascular normalization with decreased vessel caliber, density, and permeability. To further resolve the tumor microvasculature, we performed correlated UM of fluorescently labeled microvessels in cleared brains. UM resolved typical features of neoangiogenesis and tumor cell invasion with a spatial resolution of ~5 µm. MR-UM can be used as a platform for three-dimensional mapping and high-resolution quantification of tumor angiogenesis.
Item Description:Gesehen am 28.11.2019
Physical Description:Online Resource
ISSN:2050-084X
DOI:10.7554/eLife.11712

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