Split intein-mediated selection of cells containing two plasmids using a single antibiotic
Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
31 October 2019
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| In: |
Nature Communications
Year: 2019, Volume: 10 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12911-1 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1038/s41467-019-12911-1 Verlag, Volltext: https://www.nature.com/articles/s41467-019-12911-1 
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| Author Notes: | Navaneethan Palanisamy, Anna Degen, Anna Morath, Jara Ballestin Ballestin, Claudia Juraske, Mehmet Ali Öztürk, Georg A. Sprenger, Jung-Won Youn, Wolfgang W. Schamel, Barbara Di Ventura |
| Summary: | Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic. |
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| Item Description: | Gesehen am 04.12.2019 |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12911-1 |