Buchumschlag

Split intein-mediated selection of cells containing two plasmids using a single antibiotic

Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Palanisamy, Navaneethan (VerfasserIn) , Degen, Anna (VerfasserIn) , Morath, Anna (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 31 October 2019
In: Nature Communications
Year: 2019, Jahrgang: 10
ISSN:2041-1723
DOI:10.1038/s41467-019-12911-1
Online-Zugang:Verlag, Volltext: https://doi.org/10.1038/s41467-019-12911-1
Verlag, Volltext: https://www.nature.com/articles/s41467-019-12911-1
Volltext
Verfasserangaben:Navaneethan Palanisamy, Anna Degen, Anna Morath, Jara Ballestin Ballestin, Claudia Juraske, Mehmet Ali Öztürk, Georg A. Sprenger, Jung-Won Youn, Wolfgang W. Schamel, Barbara Di Ventura
Beschreibung
Zusammenfassung:Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.
Beschreibung:Gesehen am 04.12.2019
Beschreibung:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-019-12911-1

Ähnliche Einträge