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Plate-based large-scale cultivation of Caenorhabditis elegans: sample preparation for the study of metabolic alterations in diabetes

Culturing Caenorhabditis elegans (C. elegans) in a large-scale manner on agar plates can be time-consuming and difficult. This protocol describes a simple and inexpensive method to obtain a large number of animals for the isolation of proteins to proceed with a western blot, mass spectrometry, or fu...

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Bibliographic Details
Main Authors: Murillo, Katharina (Author) , Fleming, Thomas (Author) , Özer, Kübra (Author) , Hammes, Hans-Peter (Author) , Morcos, Michael (Author) , Schlotterer, Andrea (Author)
Format: Article (Journal) Video
Language:English
Published: 8/24/2018
In: JoVE. Video journal
Year: 2018, Issue: 138, Pages: 1-8
ISSN:1940-087X
DOI:10.3791/58117
Subjects:
Film
Online Access:Resolving-System, Volltext: https://doi.org/10.3791/58117
Verlag: https://www.jove.com/video/58117/plate-based-large-scale-cultivation-caenorhabditis-elegans-sample
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Author Notes:Katharina Kohl, Thomas Fleming, Kübra Acunman, Hans-Peter Hammes, Michael Morcos, Andrea Schlotterer
Description
Summary:Culturing Caenorhabditis elegans (C. elegans) in a large-scale manner on agar plates can be time-consuming and difficult. This protocol describes a simple and inexpensive method to obtain a large number of animals for the isolation of proteins to proceed with a western blot, mass spectrometry, or further proteomics analyses. Furthermore, an increase of nematode numbers for immunostainings and the integration of multiple analyses under the same culturing conditions can easily be achieved. Additionally, a transfer between plates with different experimental conditions is facilitated.
Item Description:Enthält auch eine Versuchsbeschreibung in Textform
Gesehen am 05.12.2019
Wissenschaftlicher Film. Deutschland. 2018
Physical Description:Online Resource
ISSN:1940-087X
DOI:10.3791/58117

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