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Salix tetrasperma roxb. extract alleviates neuropathic pain in rats via modulation of the NF-κB/TNF-α/NOX/iNOS pathway

Patients with neuropathic pain experience chronic painful tingling, burning, and prickling sensations accompanied with hyperalgesia and/or allodynia. In this study, 38 secondary metabolites of a methanol extract from Salix tetrasperma flowers were identified by liquid chromatography-mass spectrometr...

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Main Authors: Sobeh, Mansour (Author) , Mahmoud, Mona F. (Author) , Rezq, Samar (Author) , Alsemeh, Amira E. (Author) , Sabry, Omar M. (Author) , Mostafa, Islam (Author) , Abdelfattah, Mohamed A. O. (Author) , Ait El-Allem, Khadija (Author) , El-Shazly, Assem M. (Author) , Yasri, Aziz (Author) , Wink, Michael (Author)
Format: Article (Journal)
Language:English
Published: 12 October 2019
In: Antioxidants
Year: 2019, Volume: 8, Issue: 10
ISSN:2076-3921
DOI:10.3390/antiox8100482
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/antiox8100482
Verlag, kostenfrei, Volltext: https://www.mdpi.com/2076-3921/8/10/482
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Author Notes:Mansour Sobeh, Mona F. Mahmoud, Samar Rezq, Amira E. Alsemeh, Omar M. Sabry, Islam Mostafa, Mohamed A. O. Abdelfattah, Khadija Ait El-Allem, Assem M. El-Shazly, Aziz Yasri and Michael Wink
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Summary:Patients with neuropathic pain experience chronic painful tingling, burning, and prickling sensations accompanied with hyperalgesia and/or allodynia. In this study, 38 secondary metabolites of a methanol extract from Salix tetrasperma flowers were identified by liquid chromatography-mass spectrometry (HPLC-MS/MS). The extract showed substantial anti-inflammatory, central and peripheral anti-nociceptive, antipyretic, and antioxidant activities in vitro and in different animal models. In the chronic constriction injury (CCI) rat model, the extract was able to attenuate and significantly relieve hyperalgesia and allodynia responses in a dose dependent manner and restore the myelin sheath integrity and Schwann cells average number in the sciatic nerve. The enzyme-linked immunosorbent assay (ELISA) showed that the extract significantly reduced the expression of various pro-inflammatory biomarkers including nuclear factor kabba B (NF-κB), tumor necrosis factor alpha (TNF-α), prostaglandin E2 (PGE2), 5-lipoxygenase (5-LOX), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and the oxidative stress biomarker NADPH oxidase 1 (NOX1), in brain stem and sciatic nerve tissues. These findings were supported by in vitro enzyme inhibition assays (COX-1, COX-2 and 5-LOX). Moreover, the extract significantly reduced p53 expression in the brain stem tissue. These findings support the use of S. tetrasperma in folk medicine to alleviate pain. It could be a promising natural product for further clinical investigations to treat inflammation, nociceptive pain and chronic neuropathic pain.
Item Description:Gesehen am 12.12.2019
Physical Description:Online Resource
ISSN:2076-3921
DOI:10.3390/antiox8100482

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