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miR-339-3p is a tumor suppressor in melanoma

Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen...

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Main Authors: Weber, Claudia (Author) , Luo, Chonglin (Author) , Hotz-Wagenblatt, Agnes (Author) , Gardyan, Adriane (Author) , Kordaß, Theresa (Author) , Holland-Letz, Tim (Author) , Osen, Wolfram (Author) , Eichmüller, Stefan B. (Author)
Format: Article (Journal)
Language:English
Published: April 15, 2016
In: Cancer research
Year: 2016, Volume: 76, Issue: 12, Pages: 3562-3571
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-15-2932
Online Access:Verlag, Volltext: https://doi.org/10.1158/0008-5472.CAN-15-2932
Verlag, Volltext: https://cancerres.aacrjournals.org/content/76/12/3562
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Author Notes:Claudia E.M. Weber, Chonglin Luo, Agnes Hotz-Wagenblatt, Adriane Gardyan, Theresa Kordaß, Tim Holland-Letz, Wolfram Osen, and Stefan B. Eichmüller
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Summary:Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3′ untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness.
Item Description:Gesehen am 13.12.2019
Physical Description:Online Resource
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-15-2932

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