Functional characterization of a drought-responsive invertase inhibitor from Maize (Zea mays L.)
Invertases (INVs) play essential roles in plant growth in response to environmental cues. Previous work showed that plant invertases can be post-translationally regulated by small protein inhibitors (INVINHs). Here, this study characterizes a proteinaceous inhibitor of INVs in maize (Zm-INVINH4). A...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
21 August 2019
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| In: |
International journal of molecular sciences
Year: 2019, Volume: 20, Issue: 17 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20174081 |
| Online Access: | Verlag, Volltext: https://doi.org/10.3390/ijms20174081 Verlag, Volltext: https://www.mdpi.com/1422-0067/20/17/4081 
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| Author Notes: | Lin Chen, Xiaohong Liu, Xiaojia Huang, Wei Luo, Yuming Long, Steffen Greiner, Thomas Rausch, Hongbo Zhao |
| Summary: | Invertases (INVs) play essential roles in plant growth in response to environmental cues. Previous work showed that plant invertases can be post-translationally regulated by small protein inhibitors (INVINHs). Here, this study characterizes a proteinaceous inhibitor of INVs in maize (Zm-INVINH4). A functional analysis of the recombinant Zm-INVINH4 protein revealed that it inhibited both cell wall and vacuolar invertase activities from maize leaves. A Zm-INVINH4::green fluorescent protein fusion experiment indicated that this protein localized in the apoplast. Transcript analysis showed that Zm-INVINH4 is specifically expressed in maize sink tissues, such as the base part of the leaves and young kernels. Moreover, drought stress perturbation significantly induced Zm-INVINH4 expression, which was accompanied with a decrease of cell wall invertase (CWI) activities and an increase of sucrose accumulation in both base parts of the leaves 2 to 7 days after pollinated kernels. In summary, the results support the hypothesis that INV-related sink growth in response to drought treatment is (partially) caused by a silencing of INV activity via drought-induced induction of Zm-INVINH4 protein. |
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| Item Description: | Gesehen am 23.12.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20174081 |