Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways
Background - Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported. - Purpose - We aimed to provide the fi...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
31 July 2019
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| In: |
Phytomedicine
Year: 2019, Volume: 64, Pages: 153061 |
| ISSN: | 1618-095X |
| DOI: | 10.1016/j.phymed.2019.153061 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.phymed.2019.153061 Verlag: http://www.sciencedirect.com/science/article/pii/S0944711319302272 
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| Author Notes: | Chatrawee Duangjan, Panthakarn Rangsinth, Xiaojie Gu, Shaoxiong Zhang, Michael Wink, Tewin Tencomnao |
| Summary: | Background - Glochidion zeylanicum (GZ), a common plant in Thailand and Eastern Asia, is rich in antioxidants. However, the possible anti-aging and oxidative stress resistance properties of GZ leaf extracts (hexane and methanol extracts) have not been reported. - Purpose - We aimed to provide the first science-based evidence of the beneficial effects of GZ on anti-aging and oxidative stress resistance in the Caenorhabditis elegans model. - Methods - The phytochemical composition of the hexane and methanol extracts were analyzed using GLC-MS and LC-MS. Fingerprinting analysis of the extract was performed by RP-HPLC. We determined total phenolics, flavonoids, and antioxidant properties via DPPH and ABTS assays. Oxidative stress resistance, anti-aging and lifespan were studied in C. elegans treated with leaf extracts. - Results - GZ leaf extracts protected the worms against oxidative stress and attenuated ROS accumulation. The expression of stress-response genes, such as SOD-3, and GST-4 were up-regulated, whereas HSP-16.2 was down-regulated after GZ treatment. The oxidative stress resistance properties of GZ possibly involved the DAF-16/FoxO and SKN-1/Nrf-2 transcription factors. GZ leaf extracts improved pharyngeal pumping function and autofluorescent pigment attenuation suggesting anti-aging properties. GZ leaf extracts modulated the lifespan extension in C. elegans. - Conclusion - This study reports novel anti-aging and antioxidant activities of GZ leaf extracts, suggesting a novel bioactivity for a medicinally important plant and supplementary drug against oxidative stress. |
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| Item Description: | Available online 31 July 2019 Gesehen am 08.01.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1618-095X |
| DOI: | 10.1016/j.phymed.2019.153061 |