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Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation

Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients w...

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Main Authors: Rafat, Neysan (Author) , Patry, Christian (Author) , Sabet, Ursula (Author) , Viergutz, Tim (Author) , Weiß, Christel (Author) , Tönshoff, Burkhard (Author) , Beck, Grietje (Author) , Schaible, Thomas (Author)
Format: Article (Journal)
Language:English
Published: 22 November 2019
In: Frontiers in Pediatrics
Year: 2019, Volume: 7
ISSN:2296-2360
DOI:10.3389/fped.2019.00490
Online Access:Verlag, Volltext: https://doi.org/10.3389/fped.2019.00490
Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fped.2019.00490/full
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Author Notes:Neysan Rafat, Christian Patry, Ursula Sabet, Tim Viergutz, Christel Weiss, Burkhard Tönshoff, Grietje Beck and Thomas Schaible
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Summary:Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients with congenital diaphragmatic hernia (CDH). Therefore, we investigated, if ECMO support impacts EPC and MSC numbers in CDH patients. Methods: Peripheral blood mononuclear cells from newborns with ECMO-dependent (n=18) and ECMO-independent CDH (n=12) and from healthy controls (n=12) were isolated. The number of EPC and MSC was identified by flowcytometry. Serum levels of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-2 were determined. Results: EPC and MSC were elevated in newborns with CDH. ECMO-dependent infants had higher EPC subpopulation counts (2,1-7,6-fold) before treatment compared to ECMO-independent infants. In the disease course, EPC and MSC subpopulation counts in ECMO-dependent infants were lower than before ECMO initiation. During ECMO, VEGF serum levels were significantly reduced (by 90,5%) and Ang2 levels significantly increased (by 74,8%). Conclusions: Our data suggest that ECMO might be associated with a rather impaired mobilization of EPC and MSC and with a depression of VEGF serum levels in newborns with CDH.
Item Description:Gesehen am 14.01.2020
Physical Description:Online Resource
ISSN:2296-2360
DOI:10.3389/fped.2019.00490

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