Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation
Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients w...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
22 November 2019
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| In: |
Frontiers in Pediatrics
Year: 2019, Jahrgang: 7 |
| ISSN: | 2296-2360 |
| DOI: | 10.3389/fped.2019.00490 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.3389/fped.2019.00490 Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fped.2019.00490/full 
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| Verfasserangaben: | Neysan Rafat, Christian Patry, Ursula Sabet, Tim Viergutz, Christel Weiss, Burkhard Tönshoff, Grietje Beck and Thomas Schaible |
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| 245 | 1 | 0 | |a Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation |c Neysan Rafat, Christian Patry, Ursula Sabet, Tim Viergutz, Christel Weiss, Burkhard Tönshoff, Grietje Beck and Thomas Schaible |
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| 520 | |a Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients with congenital diaphragmatic hernia (CDH). Therefore, we investigated, if ECMO support impacts EPC and MSC numbers in CDH patients. Methods: Peripheral blood mononuclear cells from newborns with ECMO-dependent (n=18) and ECMO-independent CDH (n=12) and from healthy controls (n=12) were isolated. The number of EPC and MSC was identified by flowcytometry. Serum levels of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-2 were determined. Results: EPC and MSC were elevated in newborns with CDH. ECMO-dependent infants had higher EPC subpopulation counts (2,1-7,6-fold) before treatment compared to ECMO-independent infants. In the disease course, EPC and MSC subpopulation counts in ECMO-dependent infants were lower than before ECMO initiation. During ECMO, VEGF serum levels were significantly reduced (by 90,5%) and Ang2 levels significantly increased (by 74,8%). Conclusions: Our data suggest that ECMO might be associated with a rather impaired mobilization of EPC and MSC and with a depression of VEGF serum levels in newborns with CDH. | ||
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| 787 | 0 | 8 | |i Errata |a Rafat, Neysan, 1979 - |t Corrigendum: Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation |d 2020 |w (DE-627)169430356X |
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