“Beige” cross talk between the immune system and metabolism
With thymic senescence the epithelial network shrinks to be replaced by adipose tissue. Transcription factor TBX-1 controls thymus organogenesis, however, the same TBX-1 has also been reported to orchestrate beige adipose tissue development. Given these different roles of TBX-1, we have assessed if...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2019
|
| In: |
Frontiers in endocrinology
Year: 2019, Jahrgang: 10 |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2019.00369 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.3389/fendo.2019.00369 Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fendo.2019.00369/full 
|
| Verfasserangaben: | Krisztina Banfai, David Ernszt, Attila Pap, Peter Bai, Kitti Garai, Djeda Belharazem, Judit E. Pongracz and Krisztian Kvell |
| Zusammenfassung: | With thymic senescence the epithelial network shrinks to be replaced by adipose tissue. Transcription factor TBX-1 controls thymus organogenesis, however, the same TBX-1 has also been reported to orchestrate beige adipose tissue development. Given these different roles of TBX-1, we have assessed if thymic TBX-1 expression persists and demonstrates this dualism during adulthood. We have also checked whether thymic adipose involution could yield beige adipose tissue. We have used adult mouse and human thymus tissue from various ages to evaluate the kinetics of TBX-1 expression, as well as mouse (TEP1) and human (1889c) thymic epithelial cells (TECs) for our studies. Electron micrographs show multi-locular lipid deposits typical of beige adipose cells. Histology staining shows the accumulation of neutral lipid deposits. qPCR measurements show persistent and/or elevating levels of beige-specific and beige-indicative markers (TBX-1, EAR-2, UCP-1, PPAR-gamma). We have performed miRNome profiling using qPCR-based QuantStudio platform and amplification-free NanoString platform. [...] |
|---|---|
| Beschreibung: | Published 18 June 2019 Gesehen am 14.01.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2019.00369 |