Cover Image

Optimized cultivation of porcine choroid plexus epithelial cells, a blood-cerebrospinal fluid barrier model, for studying granulocyte transmigration

A porcine in vitro model of the blood-cerebrospinal fluid barrier based on choroid plexus epithelial cells was optimized for culture on cell culture filter inserts to prevent growth of the cells through filter pores. The optimized model retained barrier function and allowed improved investigation of...

Full description

Saved in:
Bibliographic Details
Main Authors: Lauer, Alexa (Author) , März, Martin (Author) , Meyer, Svenja (Author) , Meurer, Marita (Author) , Buhr, Nicole de (Author) , Borkowski, Julia (Author) , Weiß, Christel (Author) , Schroten, Horst (Author) , Schwerk, Christian (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: Laboratory investigation
Year: 2018, Volume: 99, Issue: 8, Pages: 1245-1255
ISSN:1530-0307
DOI:10.1038/s41374-019-0250-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41374-019-0250-9
Verlag, Volltext: https://www.nature.com/articles/s41374-019-0250-9
Get full text
Author Notes:Alexa N. Lauer, Martin März, Svenja Meyer, Marita Meurer, Nicole de Buhr, Julia Borkowski, Christel Weiß, Horst Schroten, Christian Schwerk
Description
Summary:A porcine in vitro model of the blood-cerebrospinal fluid barrier based on choroid plexus epithelial cells was optimized for culture on cell culture filter inserts to prevent growth of the cells through filter pores. The optimized model retained barrier function and allowed improved investigation of porcine granulocyte transmigration across the cell layer.
Item Description:Gesehen am 16.01.2020
Received: 15 November 2018
Physical Description:Online Resource
ISSN:1530-0307
DOI:10.1038/s41374-019-0250-9

Similar Items