Optimized cultivation of porcine choroid plexus epithelial cells, a blood-cerebrospinal fluid barrier model, for studying granulocyte transmigration
A porcine in vitro model of the blood-cerebrospinal fluid barrier based on choroid plexus epithelial cells was optimized for culture on cell culture filter inserts to prevent growth of the cells through filter pores. The optimized model retained barrier function and allowed improved investigation of...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2019
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| In: |
Laboratory investigation
Year: 2018, Jahrgang: 99, Heft: 8, Pages: 1245-1255 |
| ISSN: | 1530-0307 |
| DOI: | 10.1038/s41374-019-0250-9 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41374-019-0250-9 Verlag, Volltext: https://www.nature.com/articles/s41374-019-0250-9 |
| Verfasserangaben: | Alexa N. Lauer, Martin März, Svenja Meyer, Marita Meurer, Nicole de Buhr, Julia Borkowski, Christel Weiß, Horst Schroten, Christian Schwerk |
| Zusammenfassung: | A porcine in vitro model of the blood-cerebrospinal fluid barrier based on choroid plexus epithelial cells was optimized for culture on cell culture filter inserts to prevent growth of the cells through filter pores. The optimized model retained barrier function and allowed improved investigation of porcine granulocyte transmigration across the cell layer. |
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| Beschreibung: | Gesehen am 16.01.2020 Received: 15 November 2018 |
| Beschreibung: | Online Resource |
| ISSN: | 1530-0307 |
| DOI: | 10.1038/s41374-019-0250-9 |