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Insights into the assembly and activation of the microtubule nucleator γ-TuRC

Here, we present the cryo-EM structure of γ-TuRC from Xenopus laevis at 4.8 Å global resolution, revealing a 14-spoked arrangement of GCPs and γ-tubulins in a partially flexible open left-handed spiral with a uniform sequence of GCP variants (Fig. 1a). Via specific interactions with other GCP prote...

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Main Authors: Liu, Peng (Author) , Župa, Erik (Author) , Neuner, Annett (Author) , Böhler, Anna (Author) , Flemming, Dirk (Author) , Ruppert, Thomas (Author) , Pfeffer, Stefan (Author) , Schiebel, Elmar (Author)
Format: Article (Journal)
Language:English
Published: 2020
In: Nature
Year: 2019, Volume: 578, Pages: 467-471
ISSN:1476-4687
DOI:10.1038/s41586-019-1896-6
Online Access:Resolving-System, Volltext: https://doi.org/10.1038/s41586-019-1896-6
Verlag: https://www.nature.com/articles/s41586-019-1896-6
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Author Notes:Peng Liu, Erik Zupa, Annett Neuner, Anna Böhler, Justus Loerke, Dirk Flemming, Thomas Ruppert, Till Rudack, Christoph Peter, Christian Spahn, Oliver J. Gruss, Stefan Pfeffer & Elmar Schiebel
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Summary:Here, we present the cryo-EM structure of γ-TuRC from Xenopus laevis at 4.8 Å global resolution, revealing a 14-spoked arrangement of GCPs and γ-tubulins in a partially flexible open left-handed spiral with a uniform sequence of GCP variants (Fig. 1a). Via specific interactions with other GCP proteins, the GCP6-specific insertion domain scaffolds the assembly of the γ-TuRC. Unexpectedly, we identified Actin as a bona fide structural component of γ-TuRC with functional relevance in MT nucleation. The γ-TuRC spiral geometry is suboptimal for MT nucleation and a controlled conformational rearrangement of the γ-TuRC is required for its activation. Collectively, our cryo-EM reconstruction provides unprecedented insights into the molecular organization, the assembly and the activation mechanism of vertebrate γ-TuRC and will serve as an important framework for the mechanistic understanding of fundamental biological processes associated with MT nucleation, e.g. meiotic and mitotic spindle formation and centriole biogensis4.
Item Description:Published online: 19 December 2019
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Physical Description:Online Resource
ISSN:1476-4687
DOI:10.1038/s41586-019-1896-6

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