Web-based design and analysis tools for CRISPR base editing
As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing to...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 December 2018
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| In: |
BMC bioinformatics
Year: 2018, Volume: 19 |
| ISSN: | 1471-2105 |
| DOI: | 10.1186/s12859-018-2585-4 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1186/s12859-018-2585-4
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| Author Notes: | Gue-Ho Hwang, Jeongbin Park, Kayeong Lim, Sunghyun Kim, Jihyeon Yu, Eunchong Yu, Sang-Tae Kim, Roland Eils, Jin-Soo Kim, Sangsu Bae |
| Summary: | As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate the use of such tools have not yet been developed. |
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| Item Description: | Gesehen am 23.01.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1471-2105 |
| DOI: | 10.1186/s12859-018-2585-4 |