The anti-aggregation holdase Hsp33 promotes the formation of folded protein structures
Holdase chaperones are known to be central to suppressing aggregation, but how they affect substrate conformations remains poorly understood. Here, we use optical tweezers to study how the holdase Hsp33 alters folding transitions within single maltose binding proteins and aggregation transitions bet...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2020
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| In: |
Biophysical journal
Year: 2020, Volume: 118, Issue: 1, Pages: 85-95 |
| ISSN: | 1542-0086 |
| DOI: | 10.1016/j.bpj.2019.10.040 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.bpj.2019.10.040 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S000634951930904X 
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| Author Notes: | Fatemeh Moayed, Sergey Bezrukavnikov, Mohsin M. Naqvi, Bastian Groitl, Claudia M. Cremers, Guenter Kramer, Kingshuk Ghosh, Ursula Jakob, Sander J. Tans |
| Summary: | Holdase chaperones are known to be central to suppressing aggregation, but how they affect substrate conformations remains poorly understood. Here, we use optical tweezers to study how the holdase Hsp33 alters folding transitions within single maltose binding proteins and aggregation transitions between maltose binding protein substrates. Surprisingly, we find that Hsp33 not only suppresses aggregation but also guides the folding process. Two modes of action underlie these effects. First, Hsp33 binds unfolded chains, which suppresses aggregation between substrates and folding transitions within substrates. Second, Hsp33 binding promotes substrate states in which most of the chain is folded and modifies their structure, possibly by intercalating its intrinsically disordered regions. A statistical ensemble model shows how Hsp33 function results from the competition between these two contrasting effects. Our findings reveal an unexpectedly comprehensive functional repertoire for Hsp33 that may be more prevalent among holdases and dispels the notion of a strict chaperone hierarchy. |
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| Item Description: | Available online 11 November 2019 Gesehen am 04.02.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1542-0086 |
| DOI: | 10.1016/j.bpj.2019.10.040 |