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Chemical screening pipeline for identification of specific plant autophagy modulators

Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield. Despite its significance, the...

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Bibliographic Details
Main Authors: Dauphinee, Adrian N (Author) , Cardoso, Catarina (Author) , Minina, Alyona (Author)
Format: Article (Journal)
Language:English
Published: November 04, 2019
In: Plant physiology
Year: 2019, Volume: 181, Issue: 3, Pages: 855-866
ISSN:1532-2548
DOI:10.1104/pp.19.00647
Online Access:Verlag, Volltext: https://doi.org/10.1104/pp.19.00647
Verlag, Volltext: http://www.plantphysiol.org/content/181/3/855
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Author Notes:Adrian N. Dauphinee, Catarina Cardoso, Kerstin Dalman, Jonas A. Ohlsson, Stina Berglund Fick, Stéphanie Robert, Glenn R. Hicks, Peter V. Bozhkov, Elena A. Minina
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Summary:Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield. Despite its significance, there is still a shortage of reliable tools modulating plant autophagy. Here, we describe the first robust pipeline for identification of specific plant autophagy-modulating compounds. Our screening protocol comprises four phases: (1) high-throughput screening of chemical compounds in cell cultures of tobacco (Nicotiana tabacum); (2) confirmation of the identified hits in planta using Arabidopsis (Arabidopsis thaliana); (3) further characterization of the effect using conventional molecular biology methods; and (4) verification of chemical specificity on autophagy in planta. The methods detailed here streamline the identification of specific plant autophagy modulators and aid in unraveling the molecular mechanisms of plant autophagy.
Item Description:Gesehen am 05.02.2020
Physical Description:Online Resource
ISSN:1532-2548
DOI:10.1104/pp.19.00647

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