A comparison of microCT and microPET for evaluating lymph node metastasis in a rat model
PurposeThe demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore,...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2016
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| In: |
Molecular imaging & biology
Year: 2015, Volume: 18, Issue: 2, Pages: 243-248 |
| ISSN: | 1860-2002 |
| DOI: | 10.1007/s11307-015-0890-0 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1007/s11307-015-0890-0 Verlag, Volltext: https://link.springer.com/article/10.1007%2Fs11307-015-0890-0 
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| Author Notes: | Paul Flechsig, Clemens Kratochwil, Arne Warth, Daniel Rath, Viktoria Eichwald, Peter E. Huber, Hans-Ulrich Kauczor, Uwe Haberkorn, Frederik L. Giesel |
| Summary: | PurposeThe demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats.ProceduresHuman lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[18F]fluoro-d-glucose ([18F]FDG). Thereafter, animals were sacrificed for histological analysis.ResultsLate phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [18F]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases.ConclusionThe combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases. |
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| Item Description: | Published Online: 2 September 2015 Gesehen am 11.02.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1860-2002 |
| DOI: | 10.1007/s11307-015-0890-0 |