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Upregulation of Tumor Susceptibility Gene 101 (TSG101) by mechanical stress in podocytes

Elevated mechanical stress in glomerular hypertension is thought to damage podocytes, the loss of which leads to development of glomerulosclerosis. Applying cDNA array analysis to mechanically stressed podocytes, we have recently identified TSG101 as a stretch-induced candidate gene among others. TS...

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Bibliographic Details
Main Authors: Sunohara, Masataka (Author) , Kriz, Wilhelm (Author) , Kränzlin, Bettina (Author) , Gretz, Norbert (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: Cellular and molecular biology
Year: 2019, Volume: 65, Issue: 1, Pages: 84-88
ISSN:1165-158X
DOI:10.14715/cmb/2019.65.1.15
Online Access:Verlag, Volltext: https://doi.org/10.14715/cmb/2019.65.1.15
Verlag: https://www.cellmolbiol.org/index.php/CMB/article/view/2260
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Author Notes:Masataka Sunohara, Wilhelm Kriz, Bettina Kränzlin, Matthias Kretzler, Norbert Gretz, Karlhans Endlich, Nicole Endlich
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Summary:Elevated mechanical stress in glomerular hypertension is thought to damage podocytes, the loss of which leads to development of glomerulosclerosis. Applying cDNA array analysis to mechanically stressed podocytes, we have recently identified TSG101 as a stretch-induced candidate gene among others. TSG101, which is part of the ESCRT-I complex, is involved in multivesicular body (MVB) formation. Here we demonstrate that TSG101 mRNA is strongly upregulated in conditionally immortalized mouse podocytes by cyclic mechanical stress. Differentiation of podocytes does not affect TSG101 mRNA levels. TSG101 immunofluorescence is distributed in a vesicular pattern in podocytes, the staining intensity being enhanced by mechanical stress. In DOCA/salt treated rats, a model of glomerular hypertension, glomerular TSG101 mRNA levels are elevated, and an increased number of MVBs is observed by electron microscopy in podocyte processes. Our data demonstrate that mechanical stress upregulates TSG101 in podocytes, suggesting that glomerular hypertension enhances sorting of cell surface proteins and their ligands into the degradative pathway in podocytes.
Item Description:Gesehen am 19.02.2020
Physical Description:Online Resource
ISSN:1165-158X
DOI:10.14715/cmb/2019.65.1.15

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