Kit-dependent discrepancy in D16S539 and general considerations for database matches
Throughout the last decade more companies have been offering multiplex PCR kits for forensic STR typing. As a consequence, it has been demonstrated, that an observed genotype may unexpectedly vary at a single locus when different STR kits have been used. Analysing STR profiles which have to be enter...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 February 2018
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| In: |
Forensic science international. Genetics
Year: 2018, Volume: 34, Pages: 148-151 |
| ISSN: | 1878-0326 |
| DOI: | 10.1016/j.fsigen.2018.02.012 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.fsigen.2018.02.012 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S1872497318301005 
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| Author Notes: | Barbara Karolina Zajac, Richard Zehner, Stefanie Scheiper, Melanie Weissenberger |
| Summary: | Throughout the last decade more companies have been offering multiplex PCR kits for forensic STR typing. As a consequence, it has been demonstrated, that an observed genotype may unexpectedly vary at a single locus when different STR kits have been used. Analysing STR profiles which have to be entered in a national database, unknown or undetected primer binding site mutations, insertions or deletions within the flanking region of STR loci may hinder matches and therefore have far-reaching consequences. The current study is a further example indicating that sequence variations in flanking regions are a common problem within STR typing which should not be underestimated. A deletion of 16 nucleotides close to the primer binding site downstream of the repeat sequence resulted in deviant genotypes at the D16S539 locus according to different STR kits used. |
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| Item Description: | Gesehen am 11.03.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1878-0326 |
| DOI: | 10.1016/j.fsigen.2018.02.012 |