Modern field emission scanning electron microscopy provides new perspectives for imaging kidney ultrastructure
Recent progress in electron microscopy (EM) techniques has opened new pathways to study renal tissue in research and pathology. Modern field emission scanning EM may be utilized to scan thin sections of resin-embedded tissue mounted on a conductive support. Here we sought to achieve automated imagin...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
22 August 2018
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| In: |
Kidney international
Year: 2018, Volume: 94, Issue: 3, Pages: 625-631 |
| ISSN: | 1523-1755 |
| DOI: | 10.1016/j.kint.2018.05.017 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.kint.2018.05.017 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0085253818303910 
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| Author Notes: | Carsten Dittmayer, Eckhard Völcker, Irene Wacker, Rasmus R. Schröder, Sebastian Bachmann |
| Summary: | Recent progress in electron microscopy (EM) techniques has opened new pathways to study renal tissue in research and pathology. Modern field emission scanning EM may be utilized to scan thin sections of resin-embedded tissue mounted on a conductive support. Here we sought to achieve automated imaging without the typical limitations of transmission EM with equivalent or superior quality. Extended areas of tissue were either imaged in two (nanotomy) or in three dimensions (volume EM) by serial-section-based array tomography. Single-beam and fast-recording multi-beam field emission scanning EM instruments were compared using perfusion-fixed rodent kidneys. High-resolution scans produced excellent images of tissue, cells, and organelles down to macromolecular complexes. Digital stitching of image tiles in both modes allowed seamless Google Earth-like zooming from overview to regions of interest at the nanoscale. Large datasets were created that can be rapidly shared between scientists of different disciplines or pathologists using open source software. Three-dimensional array tomography of thin sections was followed by segmentation to visualize selected features in a large volume. Furthermore, correlative light-EM enabled the identification of functional information in a structural context. Thus, limitations in biomedical transmission EM can be overcome by introducing field emission scanning EM-based technology that permits high-quality, large field-of-view nanotomy, volume EM, and correlative light-EM modes. Advantages of virtual microscopy in clinical and experimental nephrology are illustrated. |
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| Item Description: | Gesehen am 17.03.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1523-1755 |
| DOI: | 10.1016/j.kint.2018.05.017 |