Report on a large animal study with Göttingen Minipigs where regenerates and controls for articular cartilage were created in a large number: focus on the conditions of the operated stifle joints and suggestions for standardized procedures
The characterization of regenerated articular cartilage (AC) can be based on various methods, as there is an unambiguous accepted criterion neither for the natural cartilage tissue nor for regenerates. Biomechanical aspects should be considered as well, leading to the need for more equivalent sample...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
December 26, 2019
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| In: |
PLOS ONE
Year: 2019, Jahrgang: 14, Heft: 12 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0224996 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1371/journal.pone.0224996 Verlag, kostenfrei, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224996 
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| Verfasserangaben: | Markus L. Schwarz, Gregor Reisig, Andy Schütte, Kristianna Becker, Susanne Serba, Elmar Forsch, Steffen Thier, Stefan Fickert, Tamara Lenz, Christel Weiß, Svetlana Hetjens, Frederic Bludau, Friederike Bothe, Wiltrud Richter, Barbara Schneider-Wald |
| Zusammenfassung: | The characterization of regenerated articular cartilage (AC) can be based on various methods, as there is an unambiguous accepted criterion neither for the natural cartilage tissue nor for regenerates. Biomechanical aspects should be considered as well, leading to the need for more equivalent samples. The aim of the study was to describe a large animal model where 8 specimens of regenerated AC can be created in one animal plus the impact of two surgeries on the welfare of the animals. The usefulness of the inclusion of a group of untreated animals (NAT) was to analyzed. Based on the histological results the conditions of the regenerates were to be described and the impact on knee joints were to be explored in terms of degenerative changes of the cartilage. The usefulness of the statistical term “effect size” (ES) will be explained with histological results. We analyzed an animal model where 8 AC regenerates were obtained from one Göttingen Minipig, on both sides of the trochleae. 60 animals were divided into 6 groups of 10 each, where the partial thickness defects in the trochlea were filled with matrices made of Collagen I with or without autologous chondrocytes or left empty over the healing periods of 24 and 48 weeks. One additional control group consisting of 10 untreated animals was used to provide untouched “external” cartilage. |
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| Beschreibung: | Gesehen am 26.03.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0224996 |