Changes of NK cell subsets with time post-transplant in peripheral blood of renal transplant recipients
Background - There is evidence that NK cells with low cytotoxicity but strong immunoregulatory characteristics contribute to good graft outcome. We attempted to investigate which NK cell subsets increase post-transplant and might affect graft function. - Method - Lymphocyte and NK cell subsets were...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 April 2018
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| In: |
Transplant immunology
Year: 2018, Volume: 49, Pages: 59-71 |
| ISSN: | 1878-5492 |
| DOI: | 10.1016/j.trim.2018.04.005 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.trim.2018.04.005 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0966327418300170 
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| Author Notes: | Li Zhu, Mostafa Aly, Haihao Wang, Hristos Karakizlis, Rolf Weimer, Christian Morath, Ruben Jeremias Kuon, Bettina Toth, Naruemol Ekpoom, Gerhard Opelz, Volker Daniel |
| Summary: | Background - There is evidence that NK cells with low cytotoxicity but strong immunoregulatory characteristics contribute to good graft outcome. We attempted to investigate which NK cell subsets increase post-transplant and might affect graft function. - Method - Lymphocyte and NK cell subsets were determined in whole blood using eight-colour-fluorescence flow cytometry in patients pre-transplant and post-transplant. In total, 31 transplant recipients were studied. - Results - When cell numbers were compared in 9 patients pre- and 6months post-transplant, post-transplant CD56dimCD16+ (p=0.011) NK cells with the phenotype CD158a+ (p=0.008), CD158e+ (p=0.038), NKG2A+ (p=0.008), NKG2D+ (p=0.011), IFNyR+ (p=0.008), perforin+ (p=0.008), granzymeB+ (p=0.008), perforin+granzymeB+ (p=0.008) and perforin-granzymeB- (p=0.021) were lower than those pre-transplant, indicating a post-transplant reduction of cytotoxic NK cells. In 28 patients NK cell subsets were analyzed with respect to time post-transplant (median 888days post-transplant). CD56dimCD16+ NK cells co-expressing CD158a (p=0.014), NKG2D (p=0.047), IL4R (p=0.038), IL10R (p=0.008) and IFNy (p=0.036) as well as CD56bright NK cells with the phenotype TGFß+ (p=0.017), TGFR+ (p=0.035), CD158a+ (p=0.042) and perforin-granzymeB- (p=0.048) increased with time post-transplant. - Conclusion - Post-transplant, cytotoxic NK cells were lower than pre-transplant and remained low, whereas NK cell subsets with potentially immunoregulatory properties increased. |
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| Item Description: | Gesehen am 02.04.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1878-5492 |
| DOI: | 10.1016/j.trim.2018.04.005 |