Genome-wide C-SWAT library for high-throughput yeast genome tagging

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing a...

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Bibliographic Details

Main Authors:	Meurer, Matthias (Author) , Duan, Yuanqiang (Author) , Sass, Ehud (Author) , Kats, Ilia (Author) , Herbst, Konrad (Author) , Buchmuller, Benjamin (Author) , Dederer, Verena (Author) , Huber, Florian (Author) , Kirrmaier, Daniel (Author) , Štefl, Martin (Author) , Van Laer, Koen (Author) , Dick, Tobias P. (Author) , Lemberg, Marius (Author) , Khmelinskii, Anton (Author) , Levy, Emmanuel D. (Author) , Knop, Michael (Author)
Format:	Article (Journal)
Language:	English
Published:	9 July 2018
In:	Nature methods Year: 2018, Volume: 15, Issue: 8, Pages: 598-600
ISSN:	1548-7105
DOI:	10.1038/s41592-018-0045-8
Online Access:	Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41592-018-0045-8
Author Notes:	Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C. Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P. Dick, Marius K. Lemberg, Anton Khmelinskii, Emmanuel D. Levy, Michael Knop