Buchumschlag

Data-driven modeling of intracellular auxin fluxes indicates a dominant role of the ER in controlling nuclear auxin uptake

In plants, the phytohormone auxin acts as a master regulator of developmental processes and environmental responses. The best characterized process in the auxin regulatory network occurs at the subcellular scale, wherein auxin mediates signal transduction into transcriptional programs by triggering...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Middleton, Alistair (VerfasserIn) , Dal Bosco, Cristina (VerfasserIn) , Chlap, Phillip (VerfasserIn) , Bensch, Robert (VerfasserIn) , Harz, Hartmann (VerfasserIn) , Ren, Fugang (VerfasserIn) , Bergmann, Stefan (VerfasserIn) , Wend, Sabrina (VerfasserIn) , Weber, Wilfried (VerfasserIn) , Hayashi, Ken-Ichiro (VerfasserIn) , Zurbriggen, Matias D. (VerfasserIn) , Uhl, Rainer (VerfasserIn) , Ronneberger, Olaf (VerfasserIn) , Palme, Klaus (VerfasserIn) , Fleck, Christian (VerfasserIn) , Dovzhenko, Alexander (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: March 13, 2018
In: Cell reports
Year: 2018, Jahrgang: 22, Heft: 11, Pages: 3044-3057
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.02.074
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2018.02.074
Volltext
Verfasserangaben:Alistair M. Middleton, Cristina Dal Bosco, Phillip Chlap, Robert Bensch, Hartmann Harz, Fugang Ren, Stefan Bergmann, Sabrina Wend, Wilfried Weber, Ken-Ichiro Hayashi, Matias D. Zurbriggen, Rainer Uhl, Olaf Ronneberger, Klaus Palme, Christian Fleck, Alexander Dovzhenko
Beschreibung
Zusammenfassung:In plants, the phytohormone auxin acts as a master regulator of developmental processes and environmental responses. The best characterized process in the auxin regulatory network occurs at the subcellular scale, wherein auxin mediates signal transduction into transcriptional programs by triggering the degradation of Aux/IAA transcriptional repressor proteins in the nucleus. However, whether and how auxin movement between the nucleus and the surrounding compartments is regulated remain elusive. Using a fluorescent auxin analog, we show that its diffusion into the nucleus is restricted. By combining mathematical modeling with time course assays on auxin-mediated nuclear signaling and quantitative phenotyping in single plant cell systems, we show that ER-to-nucleus auxin flux represents a major subcellular pathway to directly control nuclear auxin levels. Our findings propose that the homeostatically regulated auxin pool in the ER and ER-to-nucleus auxin fluxes underpin auxin-mediated downstream responses in plant cells.
Beschreibung:Gesehen am 15.04.2020
Beschreibung:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.02.074

Ähnliche Einträge