Immuno-iocalization of ESCRT proteins in virus-infected cells by fuorescence and electron microscopy
Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, we introduce methods for detecting several ESCRT subunit proteins in virus-infected cells b...
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| Main Author: | |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
28 June 2019
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| In: |
The ESCRT complexes
Year: 2019, Pages: 73-92 |
| DOI: | 10.1007/978-1-4939-9492-2_6 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-4939-9492-2_6
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| Author Notes: | Keisuke Tabata, Atsuki Nara, Hiroko Omori, Eiji Morita |
| Summary: | Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, we introduce methods for detecting several ESCRT subunit proteins in virus-infected cells by immunofluorescence microscopy and immunoelectron microscopy (immuno-EM). We also introduce a new method; correlative light microscopy and electron microscopy (CLEM), which allows the observation of target structures with both high-resolution EM and fluorescence labeling. |
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| Item Description: | Gesehen am 16.04.2020 |
| Physical Description: | Online Resource |
| ISBN: | 9781493994922 |
| DOI: | 10.1007/978-1-4939-9492-2_6 |