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CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification

Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstr...

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Main Authors: Friedel, Thorsten (Author) , Jung-Klawitter, Sabine (Author) , Sebe, Attila (Author) , Schenk, Franziska (Author) , Modlich, Ute (Author) , Ivics, Zoltán (Author) , Schumann, Gerald G. (Author) , Buchholz, Christian J. (Author) , Schneider, Irene C. (Author)
Format: Article (Journal)
Language:English
Published: 9 March 2016
In: Stem Cells and Development
Year: 2016, Volume: 25, Issue: 9, Pages: 729-739
ISSN:1557-8534
DOI:10.1089/scd.2015.0386
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1089/scd.2015.0386
Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/scd.2015.0386
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Author Notes:Thorsten Friedel, Sabine Jung-Klawitter, Attila Sebe, Franziska Schenk, Ute Modlich, Zoltán Ivics, Gerald G. Schumann, Christian J. Buchholz and Irene C. Schneider
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Summary:Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstrate that CD30 expression is restricted to SSEA4high cells of human iPSC cultures and a human embryonic stem cell line. When CD30-LV was added to iPSCs during routine cultivation, efficient and exclusive transduction of cells positive for the pluripotency marker Oct-4 was achieved, while retaining their pluripotency. When added during the reprogramming process, CD30-LV solely transduced cells that became fully reprogrammed iPSCs as confirmed by co-expression of endogenous Nanog and the reporter gene. Thus, CD30-LV may serve as novel tool for the selective gene transfer into PSCs with broad applications in basic and therapeutic research.
Item Description:Gesehen am 21.04.2020
Physical Description:Online Resource
ISSN:1557-8534
DOI:10.1089/scd.2015.0386

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