CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification
Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstr...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
9 March 2016
|
| In: |
Stem Cells and Development
Year: 2016, Jahrgang: 25, Heft: 9, Pages: 729-739 |
| ISSN: | 1557-8534 |
| DOI: | 10.1089/scd.2015.0386 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1089/scd.2015.0386 Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/scd.2015.0386 
|
| Verfasserangaben: | Thorsten Friedel, Sabine Jung-Klawitter, Attila Sebe, Franziska Schenk, Ute Modlich, Zoltán Ivics, Gerald G. Schumann, Christian J. Buchholz and Irene C. Schneider |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1695272595 | ||
| 003 | DE-627 | ||
| 005 | 20220818045606.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200421s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/scd.2015.0386 |2 doi | |
| 035 | |a (DE-627)1695272595 | ||
| 035 | |a (DE-599)KXP1695272595 | ||
| 035 | |a (OCoLC)1341315787 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Friedel, Thorsten |d 1985- |e VerfasserIn |0 (DE-588)1103497375 |0 (DE-627)861247280 |0 (DE-576)470626216 |4 aut | |
| 245 | 1 | 0 | |a CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification |c Thorsten Friedel, Sabine Jung-Klawitter, Attila Sebe, Franziska Schenk, Ute Modlich, Zoltán Ivics, Gerald G. Schumann, Christian J. Buchholz and Irene C. Schneider |
| 264 | 1 | |c 9 March 2016 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 21.04.2020 | ||
| 520 | |a Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstrate that CD30 expression is restricted to SSEA4high cells of human iPSC cultures and a human embryonic stem cell line. When CD30-LV was added to iPSCs during routine cultivation, efficient and exclusive transduction of cells positive for the pluripotency marker Oct-4 was achieved, while retaining their pluripotency. When added during the reprogramming process, CD30-LV solely transduced cells that became fully reprogrammed iPSCs as confirmed by co-expression of endogenous Nanog and the reporter gene. Thus, CD30-LV may serve as novel tool for the selective gene transfer into PSCs with broad applications in basic and therapeutic research. | ||
| 700 | 1 | |a Jung-Klawitter, Sabine |d 1979- |e VerfasserIn |0 (DE-588)133247821 |0 (DE-627)539315338 |0 (DE-576)299723895 |4 aut | |
| 700 | 1 | |a Sebe, Attila |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schenk, Franziska |e VerfasserIn |4 aut | |
| 700 | 1 | |a Modlich, Ute |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ivics, Zoltán |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schumann, Gerald G. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Buchholz, Christian J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schneider, Irene C. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Stem Cells and Development |d Larchmont, NY : Liebert, 2004 |g 25(2016), 9, Seite 729-739 |h Online-Ressource |w (DE-627)385193378 |w (DE-600)2142305-2 |w (DE-576)27507692X |x 1557-8534 |7 nnas |a CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification |
| 773 | 1 | 8 | |g volume:25 |g year:2016 |g number:9 |g pages:729-739 |g extent:11 |a CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification |
| 856 | 4 | 0 | |u https://doi.org/10.1089/scd.2015.0386 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.liebertpub.com/doi/10.1089/scd.2015.0386 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200421 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 133247821 |a Jung-Klawitter, Sabine |m 133247821:Jung-Klawitter, Sabine |d 910000 |e 910000PJ133247821 |k 0/910000/ |p 2 | ||
| 999 | |a KXP-PPN1695272595 |e 3627711076 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1695272595","note":["Gesehen am 21.04.2020"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification","title":"CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification"}],"relHost":[{"origin":[{"publisherPlace":"Larchmont, NY","dateIssuedKey":"2004","publisher":"Liebert","dateIssuedDisp":"2004-"}],"id":{"issn":["1557-8534"],"eki":["385193378"],"zdb":["2142305-2"]},"physDesc":[{"extent":"Online-Ressource"}],"disp":"CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modificationStem Cells and Development","language":["eng"],"title":[{"title_sort":"Stem Cells and Development","title":"Stem Cells and Development"}],"pubHistory":["13.2004 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"volume":"25","issue":"9","text":"25(2016), 9, Seite 729-739","year":"2016","extent":"11","pages":"729-739"},"recId":"385193378"}],"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"9 March 2016"}],"name":{"displayForm":["Thorsten Friedel, Sabine Jung-Klawitter, Attila Sebe, Franziska Schenk, Ute Modlich, Zoltán Ivics, Gerald G. Schumann, Christian J. Buchholz and Irene C. Schneider"]},"id":{"doi":["10.1089/scd.2015.0386"],"eki":["1695272595"]},"physDesc":[{"extent":"11 S."}],"language":["eng"],"person":[{"family":"Friedel","role":"aut","roleDisplay":"VerfasserIn","given":"Thorsten","display":"Friedel, Thorsten"},{"family":"Jung-Klawitter","given":"Sabine","role":"aut","roleDisplay":"VerfasserIn","display":"Jung-Klawitter, Sabine"},{"display":"Sebe, Attila","given":"Attila","role":"aut","roleDisplay":"VerfasserIn","family":"Sebe"},{"given":"Franziska","roleDisplay":"VerfasserIn","role":"aut","display":"Schenk, Franziska","family":"Schenk"},{"display":"Modlich, Ute","role":"aut","roleDisplay":"VerfasserIn","given":"Ute","family":"Modlich"},{"family":"Ivics","given":"Zoltán","roleDisplay":"VerfasserIn","role":"aut","display":"Ivics, Zoltán"},{"display":"Schumann, Gerald G.","given":"Gerald G.","roleDisplay":"VerfasserIn","role":"aut","family":"Schumann"},{"family":"Buchholz","roleDisplay":"VerfasserIn","role":"aut","given":"Christian J.","display":"Buchholz, Christian J."},{"display":"Schneider, Irene C.","roleDisplay":"VerfasserIn","role":"aut","given":"Irene C.","family":"Schneider"}]} | ||
| SRT | |a FRIEDELTHOCD30RECEPT9201 | ||