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STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles

The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structur...

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Main Authors: Vlijm, Rifka (Author) , Li, Xue (Author) , Panić, Marko (Author) , Rüthnick, Diana (Author) , Hata, Shoji (Author) , Herrmannsdörfer, Frank (Author) , Kuner, Thomas (Author) , Heilemann, Mike (Author) , Engelhardt, Johann (Author) , Hell, Stefan (Author) , Schiebel, Elmar (Author)
Format: Article (Journal)
Language:English
Published: February 20, 2018
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2018, Volume: 115, Issue: 10, Pages: E2246-E2253
ISSN:1091-6490
DOI:10.1073/pnas.1716840115
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1716840115
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/115/10/E2246
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Author Notes:Rifka Vlijm, Xue Li, Marko Panic, Diana Rüthnick, Shoji Hata, Frank Herrmannsdörfer, Thomas Kuner, Mike Heilemann, Johann Engelhardt, Stefan W. Hell, and Elmar Schiebel
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Summary:The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structure of the centrosome linker. Here, we used stimulated emission depletion (STED) microscopy to show that each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers.
Item Description:Gesehen am 23.04.2020
Physical Description:Online Resource
ISSN:1091-6490
DOI:10.1073/pnas.1716840115

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