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K-Ras and cyclooxygenase-2 coactivation augments intraductal papillary mucinous neoplasm and Notch1 mimicking human pancreas lesions

Mutational activation of K-Ras is an initiating event of pancreatic ductal adenocarcinomas (PDAC) that may develop either from pancreatic intraepithelial neoplasia (PanIN) or intraductal papillary mucinous neoplasms (IPMN). Cyclooxygenase-2 (COX-2)-derived prostaglandin E2 (PGE2) is causally related...

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Main Authors: Chiblak, Sara (Author) , Steinbauer, Brigitte (Author) , Pohl-Arnold, Andrea (Author) , Kucher, Dagmar (Author) , Abdollahi, Amir (Author) , Schwager, Christian (Author) , Höft, Birgit (Author) , Esposito, Irene (Author) , Müller-Decker, Karin (Author)
Format: Article (Journal)
Language:English
Published: 6 July 2016
In: Scientific reports
Year: 2016, Volume: 6
ISSN:2045-2322
DOI:10.1038/srep29455
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep29455
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep29455
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Author Notes:Sara Chiblak, Brigitte Steinbauer, Andrea Pohl-Arnold, Dagmar Kucher, Amir Abdollahi, Christian Schwager, Birgit Höft, Irene Esposito & Karin Müller-Decker
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Summary:Mutational activation of K-Ras is an initiating event of pancreatic ductal adenocarcinomas (PDAC) that may develop either from pancreatic intraepithelial neoplasia (PanIN) or intraductal papillary mucinous neoplasms (IPMN). Cyclooxygenase-2 (COX-2)-derived prostaglandin E2 (PGE2) is causally related to pancreatic carcinogenesis. Here, we deciphered the impact of COX-2, a key modulator of inflammation, in concert with active mutant K-RasG12D on tumor burden and gene expression signature using compound mutant mouse lines. Concomitant activation of COX-2 and K-RasG12D accelerated the progression of pancreatic intraepithelial lesions predominantly with a cystic papillary phenotype resembling human IPMN. Transcriptomes derived from laser capture microdissected preneoplastic lesions of single and compound mutants revealed a signature that was significantly enriched in Notch1 signaling components. In vitro, Notch1 signaling was COX-2-dependent. In line with these findings, human IPMN stratified into intestinal, gastric and pancreatobillary types displayed Notch1 immunosignals with high prevalence, especially in the gastric lesions. In conclusion, a yet unknown link between activated Ras, protumorigenic COX-2 and Notch1 in IPMN onset was unraveled.
Item Description:Gesehen am 30.04.2020
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/srep29455

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