Isolation of the protein and RNA content of active sites of transcription from mammalian cells
Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content.
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
25 February 2016
|
| In: |
Nature protocols
Year: 2016, Jahrgang: 11, Heft: 3, Pages: 553-565 |
| ISSN: | 1750-2799 |
| DOI: | 10.1038/nprot.2016.032 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nprot.2016.032 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nprot.2016.032 |
| Verfasserangaben: | Svitlana Melnik, Maïwen Caudron-Herger, Lilija Brant, Ian M. Carr, Karsten Rippe, Peter R Cook & Argyris Papantonis |
| Zusammenfassung: | Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content. |
|---|---|
| Beschreibung: | Gesehen am 05.05.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1750-2799 |
| DOI: | 10.1038/nprot.2016.032 |