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Foot-and-mouth disease virus replicates independently of phosphatidylinositol 4-phosphate and type III phosphatidylinositol 4-kinases

Picornaviruses form replication complexes in association with membranes in structures called replication organelles. Common themes to emerge from studies of picornavirus replication are the need for cholesterol and phosphatidylinositol 4-phosphate (PI4P). In infected cells, type III phosphatidylinos...

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Bibliographic Details
Main Authors: Berryman, Stephen (Author) , Moffat, Katy (Author) , Harak, Christian (Author) , Lohmann, Volker (Author) , Jackson, Terry (Author)
Format: Article (Journal)
Language:English
Published: 01 August 2016
In: Journal of general virology
Year: 2016, Volume: 97, Issue: 8, Pages: 1841-1852
ISSN:1465-2099
DOI:10.1099/jgv.0.000485
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1099/jgv.0.000485
Verlag, lizenzpflichtig, Volltext: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000485
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Author Notes:Stephen Berryman, Katy Moffat, Christian Harak, Volker Lohmann, and Terry Jackson
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Summary:Picornaviruses form replication complexes in association with membranes in structures called replication organelles. Common themes to emerge from studies of picornavirus replication are the need for cholesterol and phosphatidylinositol 4-phosphate (PI4P). In infected cells, type III phosphatidylinositol 4-kinases (PI4KIIIs) generate elevated levels of PI4P, which is then exchanged for cholesterol at replication organelles. For the enteroviruses, replication organelles form at Golgi membranes in a process that utilizes PI4KIIIβ. Other picornaviruses, for example the cardioviruses, are believed to initiate replication at the endoplasmic reticulum and subvert PI4KIIIα to generate PI4P. Here we investigated the role of PI4KIII in foot-and-mouth disease virus (FMDV) replication. Our results showed that, in contrast to the enteroviruses and the cardioviruses, FMDV replication does not require PI4KIII (PI4KIIIα and PI4KIIIβ), and PI4P levels do not increase in FMDV-infected cells and PI4P is not seen at replication organelles. These results point to a unique requirement towards lipids at the FMDV replication membranes.,
Item Description:Gesehen am 07.05.2020
Physical Description:Online Resource
ISSN:1465-2099
DOI:10.1099/jgv.0.000485

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