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Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of targe...

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Bibliographic Details
Main Authors: Kellner, Nikola (Author) , Schwarz, Johannes (Author) , Sturm, Miriam (Author) , Fernandez-Martinez, Javier (Author) , Griesel, Sabine (Author) , Zhang, Wenzhu (Author) , Chait, Brian T. (Author) , Rout, Michael P. (Author) , Kück, Ulrich (Author) , Hurt, Ed (Author)
Format: Article (Journal)
Language:English
Published: 11 February 2016
In: Scientific reports
Year: 2016, Volume: 6
ISSN:2045-2322
DOI:10.1038/srep20937
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep20937
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep20937
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Author Notes:Nikola Kellner, Johannes Schwarz, Miriam Sturm, Javier Fernandez-Martinez, Sabine Griesel, Wenzhu Zhang, Brian T. Chait, Michael P. Rout, Ulrich Kück & Ed Hurt
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Summary:We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emphasis on the nuclear pore complex, holding great potential for applications in basic science and biotechnology.
Item Description:Gesehen am 11.05.2020
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/srep20937

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