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Modified STAP conditions facilitate bivalent fate decision between pluripotency and apoptosis in Jurkat T-lymphocytes

Low extracellular pH (pHe) is not only the result of cancer metabolism, but a factor of anti-cancer drug efficacy and cancer immunity. In this study, the consequences of acidic stress were evaluated by applying STAP protocol on Jurkat T-lymphocytes (2.0 × 106 cells/ml, 25 min in 37 °C). We detected...

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Hauptverfasser: Kim, Jee Young (VerfasserIn) , Cheng, Xinlai (VerfasserIn) , Alborzinia, Hamed (VerfasserIn) , Wölfl, Stefan (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 10 March 2016
In: Biochemical and biophysical research communications
Year: 2016, Jahrgang: 472, Heft: 4, Pages: 585-591
ISSN:1090-2104
DOI:10.1016/j.bbrc.2016.03.031
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.bbrc.2016.03.031
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X16303448
Volltext
Verfasserangaben:Jee Young Kim, Xinlai Cheng, Hamed Alborzinia, Stefan Wölfl (Institute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University)
Beschreibung
Zusammenfassung:Low extracellular pH (pHe) is not only the result of cancer metabolism, but a factor of anti-cancer drug efficacy and cancer immunity. In this study, the consequences of acidic stress were evaluated by applying STAP protocol on Jurkat T-lymphocytes (2.0 × 106 cells/ml, 25 min in 37 °C). We detected apoptotic process exclusively in pH 3.3 treated cells within 8 h with western blotting (WB). This programmed cell death led to significant drop of cell viability in 72 h measured by MTT assay resulting PI positive population on flow cytometry (FCM) at day 7. Quantified RT-PCR (qRT-PCR) data indicated that all of above mentioned responses are irrelevant to expression of OCT4 gene variants. Interestingly enough, pluripotent cells represented by positive alkaline phosphatase (AP) staining survived acidic stress and consequently proportion of AP positive cells was significantly increased after pH 3.3 treatment (day 7). In general, acidic treatment led to an apoptotic condition for Jurkat T-lymphocytes, which occurred independent of OCT4 induction.
Beschreibung:Gesehen am 13.05.2020
Beschreibung:Online Resource
ISSN:1090-2104
DOI:10.1016/j.bbrc.2016.03.031

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